Diagnosis of Aspergillus Infection

Aspergillosis for patients meeting Rome 2010.
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Articles Author
  Treatment of Aspergillosis: Clinical Practice Guidelines of the Infectious Diseases Society of America Thomas J. Walsh, et.al, 2008
  Antifungal susceptibility testing in Aspergillus spp. according to EUCAST methodology. Lass-Florl C et al., 2007
  Aspergillosis & Other Filamentous Fungi: Shortcomings of the Oldů & Solutions in the New Edition! Patterson, 2005
  Endemic mycoses in immune compromised patients. Bradley, 2005
  Antigen detection in the diagnosis of Invasive Aspergillosis Verweij, 2005
  Criteria for enrollment: Randomized comparison of Voriconazole and Amphotericin B in primary therapy of invasive aspergillosis Denning et al, 2001
  Risk factors for the development of invasive fungal infections - a flow diagram illustrating susceptibility to invasive Candida and Aspergillus infections (PDF) Anon, 2001
  Entry criteria for invasive aspergillosis in salvage caspofungin study Maertens et al, 2000
Articles Author
Allergic bronchopulmonary aspergillosis in cystic fibrosis - state of the art: Cystic Fibrosis Foundation Consensus Conference Stevens et.el, 2010
Revised Definitions of Invasive Fungal Disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/M De Pauw et al., 2008
Public Consultation Document: EORTC/BAMSG Consensus Revised definitions draft VI Proven invasive fungal diseases Deep tissue disease Moulds EORTC/MSG, 2005
EORTC revised definitions II. Why revise? Process and Definitions. Donnelly, 2005
Published rather than Proposed Definitions for Invasive Fungal Infection Must Be Applied to Allow Standardization in Clinical Trials McLintock et al, 2004
Analysis of Definitions Used In Clinical Research on Invasive Fungal Infections (IFI): Consensus Proposal for New, Standardized Definitions. Ascoiglu et al, 2003
Defining Opportunistic Invasive Fungal Infections in Immunocompromised Patients with Cancer and Hematopoietic Stem Cell Transplants: An International Consensus Ascioglu et al, 2002
Selected papers on diagnostic tests:
Blood (4 categories).
Blood 9 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
qPCR 5cfu/mL
100
100
Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System, Loeffler et al, 2000
PCR NR
100
84
Early Detection of Aspergillus Infection after Allogeneic Stem Cell Transplantation by Polymerase Chain Reaction Screening, Herbart et al, 2000b
PCR NR
100
73
Prospective screening by a panfungal polymerase chain reaction assay in patients at risk for fungal infections: implications for the management of febrile neutropenia, Hebart et al, 2000
rtPCR 20 copies/mL
79
92
Use of Real-Time PCR on Blood Samples for Diagnosis of Invasive Aspergillosis, Kami et al, 2001
rtPCR 20pg/mL
67
84
Use of Real-Time PCR on Blood Samples for Diagnosis of Invasive Aspergillosis, Kami et al, 2001
Galactomannan (ELISA) >=1.5
58
97
Use of Real-Time PCR on Blood Samples for Diagnosis of Invasive Aspergillosis, Kami et al, 2001
PCR-ELISA 10cfu/mL
25
NR
Polymerase Chain Reaction Detection of Aspergillus DNA in Experimental Models of Invasive Aspergillosis, Loeffler et al, 2002b
PCR >1.0cfu/mL
NR
100
Automated Extraction of Genomic DNA from Medically Important Yeast Species and Filamentous Fungi by Using the MagNA Pure LC System, Loeffler, 2002
PCR NR
NR
NR
Detection of medically important fungi by Polymerase chain reaction, Loeffler, 2001
Plasma 2 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (Latex agglutination) NR
97.6
96.2
Detection of circulating galactomannan by Pastorex Aspergillus in experimental invasive aspergillosis., Van Cutsem et al, 1990
▀-Glucan 20 pg/mL
90
100
Plasma (1-->3)-beta-D-glucan measurement in diagnosis of invasive deep mycosis and fungal febrile episodes, Obayashi et al, 1995
Serum 90 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
100
100
Detection of Antigen in Sera of Patients with Invasive Aspergillosis: Intra- and Interlaboratory Reproducibility, Verweij et al, 1998
Galactomannan (ELISA) 0.6
100
93
Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (133)--D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorde, Kawazu et al, 2004
Galactomannan (ELISA) NR
100
93
Detection by ELISA of Aspergillus galactomannan in sera from hematological patients (ECMM Meeting Abstract)., Poirot et al, 1996
▀-Glucan 60pg/mL
100
90
b-d-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome, Odabasi et al, 2004j
▀-Glucan >=60pg/mL
100
90
b-d-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome, Odabasi et al, 2004
Galactomannan (ELISA) NR
100
89
Value of galactomannan detection by ELISA in the diagnosis and follow-up of invasive aspergillosis. , Tabone et al, 1997
Galactomannan (ELISA) NR
100
89
Improved management of invasive aspergillosis in neutropenic patients using early thoracic computed tomographic scan and surgery., Caillot et al, 1997
Galactomannan (ELISA) 0.6
100
55
Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (133)--D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorde, Kawazu et al, 2004
▀-Glucan 60pg/mL
100
NR
Advances in the serological diagnosis of invasive Aspergillus infections in patients with haematological disorders, Maertens et al, 2007
Galactomannan (ELISA) NA
100
NR
False-positive results in premature infants with the Platelia Aspergillus sandwich enzyme-linked immunosorbent assay., Siemann, 1998
Galactomannan (ELISA) >=0.5 OD
97.4
90.5
Optimization of the cutoff value for the Aspergillus double-sandwich enzyme immunoassay., Maertens et al, 2007b
Galactomannan (ELISA) >=0.5
96.5
98.6
Prospective clinical evaluation of lower cut-offs for galactomannan detection in adult neutropenic cancer patients and haematological stem cell transplant recipients, Maertens et al, 2004
Galactomannan (ELISA) >=0.7
96.5
93.2
Prospective clinical evaluation of lower cut-offs for galactomannan detection in adult neutropenic cancer patients and haematological stem cell transplant recipients, Maertens et al, 2004
Galactomannan (ELISA) NR
95
100
The diagnosis of invasive aspergillosis by an enzyme-linked immunosorbent assay for circulating antigen., Sabetta et al, 1985
Galactomannan + antibody to A. fumigatus NR
95
90
Value of antigen and antibody detection in the serological diagnosis of invasive aspergillosis in patients with hematological malignancies., Manso et al, 1994
Galactomannan (ELISA) >=1.0
94.4
98.8
Use of Circulating Galactomannan Screening for Early Diagnosis of Invasive Aspergillosis in Allogeneic Stem Cell Transplant Recipients, Maertens et al, 2002
Galactomannan (ELISA) >=1.0
94.4
85.4
Use of Circulating Galactomannan Screening for Early Diagnosis of Invasive Aspergillosis in Allogeneic Stem Cell Transplant Recipients, Maertens et al, 2002
Galactomannan (ELISA) >=1.0
93.1
100
Prospective clinical evaluation of lower cut-offs for galactomannan detection in adult neutropenic cancer patients and haematological stem cell transplant recipients, Maertens et al, 2004
▀-Glucan >=80pg/mL
92.9
100
Evaluation of a (1->3)-beta-D-Glucan Assay for Diagnosis of Invasive Fungal Infection, Pickering et al, 2005
Galactomannan (ELISA) >=1.0
92.6
95.4
Autopsy-Controlled Prospective Evaluation of Serial Screening for Circulating Galactomannan by a Sandwich Enzyme-Linked Immunosorbent Assay for Hematological Patients at Risk for Invasive Aspergillosis, Maertens et al, 1999
Galactomannan (ELISA) 1ng/mL
92.6
95.4
Autopsy-Controlled Prospective Evaluation of Serial Screening for Circulating Galactomannan by a Sandwich Enzyme-Linked Immunosorbent Assay for Hematological Patients at Risk for Invasive Aspergillosis, Maertens et al, 1999
Galactomannan (ELISA) NR
92.6
94.5
Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment for hematological malignancies., Verweij et al, 1995c
PCR NR
92.3
94.6
The Evolution and Evaluation of a Whole Blood Polymerase Chain Reaction Assay for the Detection of Invasive Aspergillosis in Hematology Patients in a Routine Clinical Setting, White et al, 2006
▀-Glucan 20pg/mL
90
100
Advances in the serological diagnosis of invasive Aspergillus infections in patients with haematological disorders, Maertens et al, 2007
Galactomannan (Latex agglutination) NR
90
84
Clinical evaluation and reproducibility of the Pastorex Aspergillus antigen latex agglutination test for diagnosing invasive aspergillosis., Verweij et al, 1995b
Galactomannan (Latex aggluination vs ELISA) NR
90
84
Sandwich Enzyme-Linked Immunosorbent Assay Compared with Pastorex Latex Agglutination Test for Diagnosing Invasive Aspergillosis in Immunocompromised Patients, Verweij et al, 1995
Galactomannan (ELISA) >=1.0
89.7
98.1
Screening for circulating galactomannan as a noninvasive diagnostic tool for invasive aspergillosis in prolonged neutropenic patients and stem cell transplantation recipients: a prospective validation, Maertens et al, 2001
▀-Glucan+Galactomannan >=1.5
87.5
100
Contribution of (133)-▀-D-Glucan Chromogenic Assay to Diagnosis and Therapeutic Monitoring of Invasive Aspergillosis in Neutropenic Adult Patients: a Comparison with Serial Screening for Circulating Galactomannan, Pazos et al, 2005
Galactomannan + ▀-Glucan NR
87.5
100
Contribution of (1->3)-?-D-glucan Chromogenic Assay to Diagnosis and Therapeutic Monitoring of Invasive Aspergillosis in Neutropenic Adult Patients: a Comparison with Serial Screening for Circulating Galactomannan., Pazos et al, 2005.
Galactomannan + ▀-Glucan >=1.5
87.5
89.6
Contribution of (133)-D-▀-Glucan Chromogenic Assay to Diagnosis and Therapeutic Monitoring of Invasive Aspergillosis in Neutropenic Adult Patients: a Comparison with Serial Screening for Circulating Galactomannan, Pazos et al, 2005
▀-Glucan >=120pg/mL
87.5
89.6
Contribution of (133)--D-Glucan Chromogenic Assay to Diagnosis and Therapeutic Monitoring of Invasive Aspergillosis in Neutropenic Adult Patients: a Comparison with Serial Screening for Circulating Galactomannan, Pazos et al, 2005
▀-Glucan >=120pg/mL
87.5
89.6
Contribution of (1->3)-?-D-glucan Chromogenic Assay to Diagnosis and Therapeutic Monitoring of Invasive Aspergillosis in Neutropenic Adult Patients: a Comparison with Serial Screening for Circulating Galactomannan., Pazos et al, 2005.
Galactomannan (ELISA) 0.5
87.5
NR
Detection of Galactomannan Antigenemia by Enzyme Immunoassay for the Diagnosis of Invasive Aspergillosis: Variables That Affect Performance, Marr et al, 2004
Galactomannan (Latex agglutination) NR
87
NR
galactomannane dans les aspergilloses invasives humaines et animales avec un test au latex., Dupont et al, 1990
Galactomannan (Latex agglutination) NR
82.5
81
Aspergillus antigenuria compared to antigenemia in bone marrow transplant recipients., Ansorg et al, 1994
Galactomannan (ELISA) >=0.5 OD
81
89
Rapid diagnosis of invasive aspergillosis by antigen detection, Wheat, 2003
Galactomannan + PCR NRd
79
61
Evaluation of pan-fungal PCR assay and Aspergillus antigen detection in the diagnosis of invasive fungal infections in high risk paediatric cancer patients, El-Mahallawy et al, 2006
PCR NR
75
92
Evaluation of pan-fungal PCR assay and Aspergillus antigen detection in the diagnosis of invasive fungal infections in high risk paediatric cancer patients, El-Mahallawy et al, 2006
Galactomannan (Latex agglutination) NR
70
86
Clinical evaluation and reproducibility of the Pastorex Aspergillus antigen latex agglutination test for diagnosing invasive aspergillosis., Verweij et al, 1995b
Galactomannan (Latex aggluination vs ELISA) NR
70
86
Sandwich Enzyme-Linked Immunosorbent Assay Compared with Pastorex Latex Agglutination Test for Diagnosing Invasive Aspergillosis in Immunocompromised Patients, Verweij et al, 1995
▀-Glucan >=60pg/mL
69.9
87.1
Multicenter Clinical Evaluation of the (1->3) b-d-Glucan Assay as an Aid to Diagnosis of Fungal Infections in Humans, Ostrosky-Zeichner et al, 2005j
▀-Glucan 60pg/mL
69.9
87.1
Multicenter Clinical Evaluation of the (1->3) ?-D-glucan Assay as an Aid to Diagnosis of Fungal Infections in Humans., Ostrosky-Zeichner et al, 2005
Galactomannan (Latex aggluination vs ELISA) NR
67
98.2
Comparison of an enzyme immunoassay and a latex agglutination system for the diagnosis of invasive aspergillosis in bone marrow transplant recipients., Machetti et al, 1998
▀-Glucan 60pg/mL
65
96
b-d-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome, Odabasi et al, 2004j
▀-Glucan >=60pg/mL
65
96
b-d-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome, Odabasi et al, 2004
▀-Glucan >=80pg/mL
64.4
92.4
Multicenter Clinical Evaluation of the (1->3) b-d-Glucan Assay as an Aid to Diagnosis of Fungal Infections in Humans, Ostrosky-Zeichner et al, 2005j
▀-Glucan 80pg/mL
64.4
92.4
Multicenter Clinical Evaluation of the (1->3) ?-D-glucan Assay as an Aid to Diagnosis of Fungal Infections in Humans., Ostrosky-Zeichner et al, 2005
NR
64
93
Detection of Circulating Aspergillus fumigatus Galactomannan: Value and Limits of the Platelia Test for Diagnosing Invasive Aspergillosis, Pinel et al, 2003
PCR-ELISA NR
63.6
89.7
Prospective Evaluation of a Polymerase Chain ReactionľELISA Targeted to Aspergillus fumigatus and Aspergillus flavus for the Early Diagnosis of Invasive Aspergillosis in Patients with Hematological Malignancies, Florent et al, 2006
▀-Glucan 60pg/mL
60
99
b-d-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome, Odabasi et al, 2004j
▀-Glucan >=60pg/mL
60
99
b-d-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome, Odabasi et al, 2004
Galactomannan (ELISA) NR
60
82
Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive aspergillosis., Maertens et al, 1999
Galactomannan (ELISA) >=1.0
55.6
93.9
Aspergillus antigenemia sandwich-enzyme immunoassay test as a serodiagnostic method for invasive aspergillosis in liver transplant recipients., Fortun et al, 2001e
▀-Glucan 11ng/mL
55
98
Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (133)--D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorde, Kawazu et al, 2004
Galactomannan - Ultrasound enhanced NR
53
NR
Highly sensitive detection of fungal antigens by ultrasound-enhanced latex agglutination., Grundy et al, 1995
>=1.0
50
99.6
Detection of Circulating Aspergillus fumigatus Galactomannan: Value and Limits of the Platelia Test for Diagnosing Invasive Aspergillosis, Pinel et al, 2003
Galactomannan (Latex aggluination vs ELISA) NR
50
94
Sandwich enzyme-linked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients., Verweij et al, 1995
Galactomannan (ELISA) >=1.0
47
93
Galactomannan detection in computerized tomography-based broncho-alveolar lavage fluid and serum in haematological patients at risk for invasive pulmonary aspergillosis, Becker et al, 2003c
▀-Glucan 11ng/mL
45
99
Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (133)--D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorde, Kawazu et al, 2004
rtPCR 40 copies/mL
45
98
Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (133)--D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorde, Kawazu et al, 2004
Galactomannan (Latex agglutination) NR
43
53
Postmortem analysis of invasive aspergillosis in a tertiary care hospital., Vogeser et al, 1997
Galactomannan (ELISA) NR
40
94
Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive aspergillosis., Maertens et al, 1999
Galactomannan (Latex agglutination) NR
38.8
95
Use of the Pastorex Aspergillus antigen latex agglutination test for the diagnosis of invasive aspergillosis., Hopwood et al, 1995
rtPCR 40 copies/mL
36
100
Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (133)--D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorde, Kawazu et al, 2004
Galactomannan (ELISA) >=0.66
30
95
Prospective Assessment of PlateliaTM Aspergillus Galactomannan Antigen for the Diagnosis of Invasive Aspergillosis in Lung Transplant Recipients, Husain et al, 2004f
Galactomannan (ELISA) >=0.5
30
93
Prospective Assessment of PlateliaTM Aspergillus Galactomannan Antigen for the Diagnosis of Invasive Aspergillosis in Lung Transplant Recipients, Husain et al, 2004f
Galactomannan (Latex agglutination) NR
27.5
100
Aspergillus antigenuria compared to antigenemia in bone marrow transplant recipients., Ansorg et al, 1994
Galactomannan (ELISA) >=1.0
20
NR
Prospective Assessment of PlateliaTM Aspergillus Galactomannan Antigen for the Diagnosis of Invasive Aspergillosis in Lung Transplant Recipients, Husain et al, 2004f
Galactomannan (Latex agglutination) NR
NR
98.7
Aspergillus antigenuria compared to antigenemia in bone marrow transplant recipients., Ansorg et al, 1994
Galactomannan (ELISA) NR
NR
NR
A poster concerning the cross reactivity of galactomannan antibody EB-2A with lipoteichoic acid of Bifidobacterium from the 2003 TIFI/ECMM conference , Mennink-Kersten, 2003
Galactomannan (ELISA) Various
NR
NR
Antigen detection in the diagnosis of Invasive Aspergillosis, Verweij, 2004 - Review article
Radioimmune assay + ELISA (Galactomannan) 108ng/mL
NR
NR
Galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits., Dupont et al, 1987
Galactomannan (ELISA) NA
NR
NR
Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis , Mennink-Kersten, 2004
Galactomannan (ELISA) >=1.5OD
NR
NR
False Positive Test for Aspergillus Antigenemia Related to Concomitant Administration of Piperacillin and Tazobactam, Sulahian et al, 2003
Galactomannan (ELISA) NA
NR
NR
Lack of evidence that false-positive Aspergillus galactomannan antigen test results are due to treatment with piperacillin-tazobactam. AND REPLIES., Adam et al, 2004
Galactomannan (ELISA) NA
NR
NR
False-Positive Aspergillus Galactomannan Antigen Test Results, Maertens et al, 2004
Galactomannan (ELISA) NR
NR
NR
Efficacy of Galactomannan Antigen in the Platelia Aspergillus Enzyme Immunoassay for Diagnosis of Invasive Aspergillosis in Liver Transplant Recipients, Kwak et al, 2003
Galactomannan (ELISA) >=1.0
NR
NR
Evaluation of Serum Sandwich Enzyme-Linked Immunosorbent Assay for Circulating Galactomannan during Caspofungin Therapy: Results from the Caspofungin Invasive Aspergillosis Study, Maertens et al, 2005
Indirect Haemagglutination Assay - 50kDa AG NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Indirect Haemagglutination Assay - Galactomannan NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Indirect Haemagglutination Assay - metabolic AG NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Enzyme Immunoassay - IgG DVV NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Enzyme Immunoassay - IgM DVV NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Enzyme Immunoassay - IgA DVV NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Complement Fixation - metabolic, Virion NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Complement Fixation - somatic, Virion NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Galactomannan (Latex agglutination) NA
NR
NR
Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis., Kappe et al, 1996
Galactomannan (ELISA)
0
0
Issues with galactomannan testing, Verweij et al., 2006
Galactomannan (ELISA)
0
0
Galactoxylomannan Does Not Exhibit Cross-Reactivity in the Platelia Aspergillus Enzyme Immunoassay, De Jesus et al., 2007
Galactomannan (ELISA) vs Halo Sign on CT Scan >=0.5
NR
NR
Galactomannan Does Not Precede Major Signs on a Pulmonary Computerized Tomographic Scan Suggestive of Invasive Aspergillosis in Patients with Hematological Malignancies, Weisser et al, 2005
Serum + Plasma 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
▀-Glucan NR
93.3
77.2
Evaluation of a (1->3)-beta-D-glucan assay for diagnosis of invasive fungal infections., Pickering et al, 2005
Other fluids (7 categories).
Test Assay cutoff Sensitivity Specificity Link
quantitative-PCR (pan-Aspergillus) <=35
100
88
Luong et. et., 2011
PCR 1-5 CFU/mL
93.9
94.4
Buchheidt et al, 2002
Galactomannan 0.5
93
88
Luong et. et., 2011
PCR NR
90
100
Sangunetti et al (2003)
Galactomannan (ELISA) 0.5
88
87
Meersseman et al. (2008)
Galactomannan (ELISA) >=1.0
85
100
Becker et al, 2003c
quantitative-PCR (Aspergillus-specific) <=40
85
96
Luong et. et., 2011
qPCR+Galactomannan NR>=1.0
82
96
Musher et al, 2004g
PCR NR
80
93
Raad et al, 2002
Galactomannan + quantitative PCR >=0.5
76
100
Musher et al, 2004g
qPCR NR
67
100
Musher et al, 2004g
Cytology NA
64
99.1
Levy et al, 1992
Galactomannan + quantitative PCR >=1.0
61
67
Musher et al, 2004g
Histology+Culture NA
58
92
Kahn et al, 1986
Culture NA
56.2
92.3
Maertens et al, 2002
Histology NA
53
95
Kahn et al, 1986
Culture NA
40
90.3
Levy et al, 1992
Cytology+Culture NA
32
99.7
Levy et al, 1992
Culture NA
23
95
Kahn et al, 1986
PCR >1.0cfu/mL
NR
100
Loeffler, 2002
PCR 1pg
NR
NR
Spreadbury et.al. (1993)
Cerebrospinal fluid 10 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
80
100
Klont, 2004 (Review article)
Indirect Haemagglutination Assay - 50kDa AG NR
NR
NR
Kappe et al, 1996
Indirect Haemagglutination Assay - Galactomannan NR
NR
NR
Kappe et al, 1996
Indirect Haemagglutination Assay - metabolic AG NA
NR
NR
Kappe et al, 1996
Enzyme Immunoassay - IgG DVV NA
NR
NR
Kappe et al, 1996
Enzyme Immunoassay - IgM DVV NA
NR
NR
Kappe et al, 1996
Enzyme Immunoassay - IgA DVV NA
NR
NR
Kappe et al, 1996
Complement Fixation - metabolic, Virion NA
NR
NR
Kappe et al, 1996
Complement Fixation - somatic, Virion NA
NR
NR
Kappe et al, 1996
Galactomannan (Latex agglutination) NA
NR
NR
Kappe et al, 1996
Cyst fluid 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
NR
NR
Klont, 2004 (Review article)
Pus 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
NR
NR
Klont, 2004 (Review article)
Serum 106 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
100
100
Verweij et al, 1998
Galactomannan (ELISA) 0.6
100
93
Kawazu et al, 2004
Galactomannan (ELISA) NR
100
93
Poirot et al, 1996
▀-Glucan 60pg/mL
100
90
Odabasi et al, 2004j
▀-Glucan >=60pg/mL
100
90
Odabasi et al, 2004
Galactomannan (ELISA) NR
100
89
Tabone et al, 1997
Galactomannan (ELISA) NR
100
89
Caillot et al, 1997
Galactomannan (ELISA) 0.6
100
55
Kawazu et al, 2004
▀-Glucan 60pg/mL
100
NR
Maertens et al, 2007
Galactomannan (ELISA) NA
100
NR
Siemann, 1998
▀-Glucan
100
0
Fungitell assay
▀-Glucan
100
0
Fungitell assay
Galactomannan (ELISA) >=0.5 OD
97.4
90.5
Maertens et al, 2007b
Galactomannan (ELISA) >=0.5
96.5
98.6
Maertens et al, 2004
Galactomannan (ELISA) >=0.7
96.5
93.2
Maertens et al, 2004
Galactomannan (ELISA) NR
95
100
Sabetta et al, 1985
Galactomannan + antibody to A. fumigatus NR
95
90
Manso et al, 1994
Galactomannan (ELISA) >=1.0
94.4
98.8
Maertens et al, 2002
Galactomannan (ELISA) >=1.0
94.4
85.4
Maertens et al, 2002
Galactomannan (ELISA) >=1.0
93.1
100
Maertens et al, 2004
▀-Glucan >=80pg/mL
92.9
100
Pickering et al, 2005
Galactomannan (ELISA) >=1.0
92.6
95.4
Maertens et al, 1999
Galactomannan (ELISA) 1ng/mL
92.6
95.4
Maertens et al, 1999
Galactomannan (ELISA) NR
92.6
94.5
Verweij et al, 1995c
PCR NR
92.3
94.6
White et al, 2006
▀-Glucan 20pg/mL
90
100
Maertens et al, 2007
Galactomannan (Latex agglutination) NR
90
84
Verweij et al, 1995b
Galactomannan (Latex aggluination vs ELISA) NR
90
84
Verweij et al, 1995
Galactomannan (ELISA) >=1.0
89.7
98.1
Maertens et al, 2001
▀-Glucan+Galactomannan >=1.5
87.5
100
Pazos et al, 2005
Galactomannan + ▀-Glucan NR
87.5
100
Pazos et al, 2005.
Galactomannan + ▀-Glucan >=1.5
87.5
89.6
Pazos et al, 2005
▀-Glucan >=120pg/mL
87.5
89.6
Pazos et al, 2005
▀-Glucan >=120pg/mL
87.5
89.6
Pazos et al, 2005.
Galactomannan (ELISA) 0.5
87.5
NR
Marr et al, 2004
Galactomannan (Latex agglutination) NR
87
NR
Dupont et al, 1990
Galactomannan (Latex agglutination) NR
82.5
81
Ansorg et al, 1994
Galactomannan (ELISA) >=0.5 OD
81
89
Wheat, 2003
Galactomannan (ELISA) 1ng/mL
80.7
89.2
Platelia Aspergillus
Galactomannan (ELISA) 1ng/mL
80.7
89.2
Platelia Aspergillus
Galactomannan + PCR NRd
79
61
El-Mahallawy et al, 2006
PCR NR
75
92
El-Mahallawy et al, 2006
Galactomannan (Latex agglutination) NR
70
86
Verweij et al, 1995b
Galactomannan (Latex aggluination vs ELISA) NR
70
86
Verweij et al, 1995
▀-Glucan >=60pg/mL
69.9
87.1
Ostrosky-Zeichner et al, 2005j
▀-Glucan 60pg/mL
69.9
87.1
Ostrosky-Zeichner et al, 2005
Galactomannan (Latex aggluination vs ELISA) NR
67
98.2
Machetti et al, 1998
▀-Glucan 60pg/mL
65
96
Odabasi et al, 2004j
▀-Glucan >=60pg/mL
65
96
Odabasi et al, 2004
▀-Glucan >=80pg/mL
64.4
92.4
Ostrosky-Zeichner et al, 2005j
▀-Glucan 80pg/mL
64.4
92.4
Ostrosky-Zeichner et al, 2005
NR
64
93
Pinel et al, 2003
PCR-ELISA NR
63.6
89.7
Florent et al, 2006
▀-Glucan 60pg/mL
60
99
Odabasi et al, 2004j
▀-Glucan >=60pg/mL
60
99
Odabasi et al, 2004
Galactomannan (ELISA) NR
60
82
Maertens et al, 1999
Galactomannan (ELISA) >=1.0
55.6
93.9
Fortun et al, 2001e
▀-Glucan 11ng/mL
55
98
Kawazu et al, 2004
Galactomannan - Ultrasound enhanced NR
53
NR
Grundy et al, 1995
>=1.0
50
99.6
Pinel et al, 2003
Galactomannan (Latex aggluination vs ELISA) NR
50
94
Verweij et al, 1995
Galactomannan (ELISA) >=1.0
47
93
Becker et al, 2003c
▀-Glucan 11ng/mL
45
99
Kawazu et al, 2004
rtPCR 40 copies/mL
45
98
Kawazu et al, 2004
Galactomannan (Latex agglutination) NR
43
53
Vogeser et al, 1997
Galactomannan (ELISA) NR
40
94
Maertens et al, 1999
Galactomannan (Latex agglutination) NR
38.8
95
Hopwood et al, 1995
rtPCR 40 copies/mL
36
100
Kawazu et al, 2004
Galactomannan (ELISA) >=0.66
30
95
Husain et al, 2004f
Galactomannan (ELISA) >=0.5
30
93
Husain et al, 2004f
Galactomannan (Latex agglutination) NR
27.5
100
Ansorg et al, 1994
Galactomannan (ELISA) >=1.0
20
NR
Husain et al, 2004f
Galactomannan (Latex agglutination) NR
NR
98.7
Ansorg et al, 1994
Galactomannan (ELISA) NR
NR
NR
Mennink-Kersten, 2003
Galactomannan (ELISA) Various
NR
NR
Verweij, 2004 - Review article
Radioimmune assay + ELISA (Galactomannan) 108ng/mL
NR
NR
Dupont et al, 1987
▀-Glucan
0
0
FungiTec G
Galactomannan (ELISA) NA
NR
NR
Mennink-Kersten, 2004
Galactomannan (ELISA) >=1.5OD
NR
NR
Sulahian et al, 2003
Galactomannan (ELISA) NA
NR
NR
Adam et al, 2004
Galactomannan (ELISA) NA
NR
NR
Maertens et al, 2004
Galactomannan (ELISA) NR
NR
NR
Kwak et al, 2003
Galactomannan (ELISA) >=1.0
NR
NR
Maertens et al, 2005
Indirect Haemagglutination Assay - 50kDa AG NA
NR
NR
Kappe et al, 1996
Indirect Haemagglutination Assay - Galactomannan NA
NR
NR
Kappe et al, 1996
Indirect Haemagglutination Assay - metabolic AG NA
NR
NR
Kappe et al, 1996
Enzyme Immunoassay - IgG DVV NA
NR
NR
Kappe et al, 1996
Enzyme Immunoassay - IgM DVV NA
NR
NR
Kappe et al, 1996
Enzyme Immunoassay - IgA DVV NA
NR
NR
Kappe et al, 1996
▀-Glucan 0-300pg/ml
0
0
Gluspecy
Complement Fixation - metabolic, Virion NA
NR
NR
Kappe et al, 1996
Complement Fixation - somatic, Virion NA
NR
NR
Kappe et al, 1996
▀-Glucan 1-10,000pg/ml
0
0
Beta-D-glucan Test Wako
Galactomannan (Latex agglutination) NA
NR
NR
Kappe et al, 1996
▀-Glucan 0-50pg/ml
0
0
B-G Star
Galactomannan (Latex agglutination) 15 ng/mL
0
0
PastorexÖ Aspergillus*
Galactomannan (ELISA)
0
0
Dalle et al., 2005
Galactomannan (ELISA)
0
0
Verweij et al., 2006
Galactomannan (ELISA)
0
0
Wu, 2004
Galactomannan (ELISA)
0
0
Mattei et al., 2004
Galactomannan (ELISA)
0
0
Aubry et al., 2006
Galactomannan (ELISA)
0
0
Sulahian et al., 2003
Galactomannan (ELISA)
0
0
De Jesus et al., 2007
Galactomannan (ELISA)
0
0
Kappe et al., 1993
Galactomannan (ELISA)
0
0
Giacchino et al., 2006
Galactomannan (ELISA) vs Halo Sign on CT Scan >=0.5
NR
NR
Weisser et al, 2005
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
NR
NR
Klont, 2004 (Review article)
Urine 3 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
44
NR
Klont, 2004 (Review article)
Radioimmune assay + ELISA (Galactomannan) 1-83 ng/mL
NR
NR
Dupont et al, 1987
Galactomannan (ELISA)
0
0
Hashiguchi et al., 1994
Solid Tissue (1 categories).
Solid tissue 3 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NR
NR
NR
Klont, 2004 (Review article)
Galactomannan (ELISA) NR
NR
NR
Laboratoire de Parasitologie-Mycologie, 2004
Respiratory (4 categories).
Bronchoalveolar lavage 21 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
quantitative-PCR (pan-Aspergillus) <=35
100
88
Luong et. et., 2011
PCR 1-5 CFU/mL
93.9
94.4
Buchheidt et al, 2002
Galactomannan 0.5
93
88
Luong et. et., 2011
PCR NR
90
100
Sangunetti et al (2003)
Galactomannan (ELISA) 0.5
88
87
Meersseman et al. (2008)
Galactomannan (ELISA) >=1.0
85
100
Becker et al, 2003c
quantitative-PCR (Aspergillus-specific) <=40
85
96
Luong et. et., 2011
qPCR+Galactomannan NR>=1.0
82
96
Musher et al, 2004g
PCR NR
80
93
Raad et al, 2002
Galactomannan + quantitative PCR >=0.5
76
100
Musher et al, 2004g
qPCR NR
67
100
Musher et al, 2004g
Cytology NA
64
99.1
Levy et al, 1992
Galactomannan + quantitative PCR >=1.0
61
67
Musher et al, 2004g
Histology+Culture NA
58
92
Kahn et al, 1986
Culture NA
56.2
92.3
Maertens et al, 2002
Histology NA
53
95
Kahn et al, 1986
Culture NA
40
90.3
Levy et al, 1992
Cytology+Culture NA
32
99.7
Levy et al, 1992
Culture NA
23
95
Kahn et al, 1986
PCR >1.0cfu/mL
NR
100
Loeffler, 2002
PCR 1pg
NR
NR
Spreadbury et.al. (1993)
Halo Sign on CT Scan 2 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Halo Sign on CT Scan NA
33.3
93.3
Maertens et al, 2002
Halo Sign on CT Scan vs Galactomannan (ELISA) >=0.5
NR
NR
Weisser et al, 2005
Test Assay cutoff Sensitivity Specificity Link
Culture NA
61.1
96.3
Maertens et al, 2002
Test Assay cutoff Sensitivity Specificity Link
Positron Emission Tomography n/a
NA
NA
Aspergillus Website, 2008
Animal model (4 categories).
Blood 10 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
qPCR 5cfu/mL
100
100
Loeffler et al, 2000
PCR NR
100
84
Herbart et al, 2000b
PCR NR
100
73
Hebart et al, 2000
rtPCR 20 copies/mL
79
92
Kami et al, 2001
rtPCR 20pg/mL
67
84
Kami et al, 2001
Galactomannan (ELISA) >=1.5
58
97
Kami et al, 2001
PCR-ELISA 10cfu/mL
25
NR
Loeffler et al, 2002b
PCR >1.0cfu/mL
NR
100
Loeffler, 2002
PCR NR
NR
NR
Loeffler, 2001
rtPCR 3 copies
0
0
LightCycler«SeptiFast Test
Test Assay cutoff Sensitivity Specificity Link
quantitative-PCR (pan-Aspergillus) <=35
100
88
Luong et. et., 2011
PCR 1-5 CFU/mL
93.9
94.4
Buchheidt et al, 2002
Galactomannan 0.5
93
88
Luong et. et., 2011
PCR NR
90
100
Sangunetti et al (2003)
Galactomannan (ELISA) 0.5
88
87
Meersseman et al. (2008)
Galactomannan (ELISA) >=1.0
85
100
Becker et al, 2003c
quantitative-PCR (Aspergillus-specific) <=40
85
96
Luong et. et., 2011
qPCR+Galactomannan NR>=1.0
82
96
Musher et al, 2004g
PCR NR
80
93
Raad et al, 2002
Galactomannan + quantitative PCR >=0.5
76
100
Musher et al, 2004g
qPCR NR
67
100
Musher et al, 2004g
Cytology NA
64
99.1
Levy et al, 1992
Galactomannan + quantitative PCR >=1.0
61
67
Musher et al, 2004g
Histology+Culture NA
58
92
Kahn et al, 1986
Culture NA
56.2
92.3
Maertens et al, 2002
Histology NA
53
95
Kahn et al, 1986
Culture NA
40
90.3
Levy et al, 1992
Cytology+Culture NA
32
99.7
Levy et al, 1992
Culture NA
23
95
Kahn et al, 1986
PCR >1.0cfu/mL
NR
100
Loeffler, 2002
PCR 1pg
NR
NR
Spreadbury et.al. (1993)
Lung 5 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
PCR-ELISA 10cfu/mL
78
NR
Loeffler et al, 2002b
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Immunohistology Mab 164G & 611F (Species specific) NA
NR
NR
Fenelon et al, 1999
Immunohistology Mab EB-A1 (Galactomannan) NA
NR
NR
Verweij et al, 2007
Rabbit immunoglobulin to A. fumigatus B-1172 NA
NR
NR
Kaufman et al, 1997
Plasma 2 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (Latex agglutination) NR
97.6
96.2
Van Cutsem et al, 1990
▀-Glucan 20 pg/mL
90
100
Obayashi et al, 1995
Sources of error (3 categories).
False Positives 12 entry(ies).
Specimen Test Link
Foods Galactomannan (ELISA) V. Letscher-Bru et al, 1998
Cardboard contamination Galactomannan (ELISA) Girmenia et al, 2007
Crossreactivity with lipoteichoic acid Galactomannan (ELISA) Mennink-Kersten, 2004
Crossreactivity with lipoteichoic acid Galactomannan (ELISA) Mennink-Kersten, 2005
Serum Galactomannan (ELISA) Dalle et al., 2005
Serum Galactomannan (ELISA) Wu, 2004
Serum Galactomannan (ELISA) Mattei et al., 2004
Serum Galactomannan (ELISA) Aubry et al., 2006
Serum Galactomannan (ELISA) Sulahian et al., 2003
Urine Galactomannan (ELISA) Hashiguchi et al., 1994
Serum Galactomannan (ELISA) Kappe et al., 1993
Serum Galactomannan (ELISA) Giacchino et al., 2006
Specimen Test Link
Serum Galactomannan (ELISA) De Jesus et al., 2007
Specimen Test Link
Serum Galactomannan (ELISA) Verweij et al., 2006
Paediatrics (8 categories).
Brain 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NA
NR
NR
Mennink-Kersten, 2004
Galactomannan (ELISA) NA
NR
NR
Mennink-Kersten, 2005
Ear 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Heart 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Lung 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Nasopharynx 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Serum 2 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Galactomannan (ELISA) NA
100
NR
Siemann, 1998
Galactomannan (ELISA) NA
NR
NR
Mennink-Kersten, 2004
Spleen 1 entry(ies).
Test Assay cutoff Sensitivity Specificity Link
Immunohistology Mab-WF-AF-1 (Galactomannan) NA
NR
NR
Choi et al, 2004
Reviews (5 categories).
General 1 entry(ies).
Title Link
Laboratory diagnosis of invasive aspergillosis Hope et al, 2005
Media 1 entry(ies).
Title Link
Antifungal susceptibility testing in Aspergillus spp. according to EUCAST methodology. Lass-Florl C et al., 2007
Serum 7 entry(ies).
Title Link
Detection of Antigen in Sera of Patients with Invasive Aspergillosis: Intra- and Interlaboratory Reproducibility Verweij et al, 1998
Advances in the serological diagnosis of invasive Aspergillus infections in patients with haematological disorders Maertens et al, 2007
Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis Mennink-Kersten, 2004
Lack of evidence that false-positive Aspergillus galactomannan antigen test results are due to treatment with piperacillin-tazobactam. AND REPLIES. Adam et al, 2004
False-Positive Aspergillus Galactomannan Antigen Test Results Maertens et al, 2004
Evaluation of Serum Sandwich Enzyme-Linked Immunosorbent Assay for Circulating Galactomannan during Caspofungin Therapy: Results from the Caspofungin Invasive Aspergillosis Study Maertens et al, 2005
Issues with galactomannan testing Verweij et al., 2006
Serum & Plasma 1 entry(ies).
Title Link
Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysis Mengoli et al, 2009
Serum + Urine + BAL 1 entry(ies).
Title Link
Advances in the serological diagnosis of invasive Aspergillus infections in patients with haematological disorders Maertens et al, 2007
Fixed samples (13 categories).
Brain 3 entry(ies).
Test Link
Immunohistology Mab-WF-AF-1 (Galactomannan) Choi et al, 2004
Immunohistology Mab 164G & 611F (Species specific) Fenelon et al, 1999
Rabbit immunoglobulin to A. fumigatus B-1172 Kaufman et al, 1997
Ear 1 entry(ies).
Test Link
Immunohistology Mab-WF-AF-1 (Galactomannan) Choi et al, 2004
Heart 1 entry(ies).
Test Link
Immunohistology Mab-WF-AF-1 (Galactomannan) Choi et al, 2004
Kidney 1 entry(ies).
Test Link
Rabbit immunoglobulin to A. fumigatus B-1172 Kaufman et al, 1997
Liver 1 entry(ies).
Test Link
Immunohistology Mab 164G & 611F (Species specific) Fenelon et al, 1999
Lung 5 entry(ies).
Test Link
PCR-ELISA Loeffler et al, 2002b
Immunohistology Mab-WF-AF-1 (Galactomannan) Choi et al, 2004
Immunohistology Mab 164G & 611F (Species specific) Fenelon et al, 1999
Immunohistology Mab EB-A1 (Galactomannan) Verweij et al, 2007
Rabbit immunoglobulin to A. fumigatus B-1172 Kaufman et al, 1997
Maxillary sinus 1 entry(ies).
Test Link
Rabbit immunoglobulin to A. fumigatus B-1172 Kaufman et al, 1997
Nasopharynx 1 entry(ies).
Test Link
Immunohistology Mab-WF-AF-1 (Galactomannan) Choi et al, 2004
Necrotic bone 1 entry(ies).
Test Link
Immunohistology Mab EB-A1 (Galactomannan) Verweij et al, 2007
Paranasal sinus 1 entry(ies).
Test Link
Immunohistology Mab 164G & 611F (Species specific) Fenelon et al, 1999
Phagocytes 1 entry(ies).
Test Link
Immunohistology Mab EB-A1 (Galactomannan) PiÚrard et al, 1991
Skin 1 entry(ies).
Test Link
Rabbit immunoglobulin to A. fumigatus B-1172 Kaufman et al, 1997
Spleen 1 entry(ies).
Test Link
Immunohistology Mab-WF-AF-1 (Galactomannan) Choi et al, 2004
Tests
 
Specimen Sensitivity Specificity Link
0
0
A. fumigatus Complement Fixation Reagents
Specimen Sensitivity Specificity Link
0
0
Labor Diagnostics
Specimen Sensitivity Specificity Link
0
0
Roche Serologie GmbH, Munich
Specimen Sensitivity Specificity Link
0
0
Kappe, 1996
Specimen Sensitivity Specificity Link
0
0
Fenelon et al., 1999
Specimen Sensitivity Specificity Link
0
0
Aspergillus antibodies (x5)
Specimen Sensitivity Specificity Link
0
0
Choi et al., 2004
Specimen Sensitivity Specificity Link
Media
0
0
Lass-Florl C et al., 2007
Specimen Sensitivity Specificity Link
0
0
Aspergillus antibodies (x32)
0
0
Aspergillus antibodies (x9)
Specimen Sensitivity Specificity Link
0
0
Aspergillus Immunodiffusion Group Species
0
0
Aspergillus Immunodiffusion Individual Species
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Culture 4 entry(ies).
Specimen Sensitivity Specificity Link
Lower respiratory tract
61.1
96.3
Maertens et al, 2002
Bronchoalveolar lavage
56.2
92.3
Maertens et al, 2002
Bronchoalveolar lavage
40
90.3
Levy et al, 1992
Bronchoalveolar lavage
23
95
Kahn et al, 1986
Cytology 1 entry(ies).
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
64
99.1
Levy et al, 1992
Cytology+Culture 1 entry(ies).
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
32
99.7
Levy et al, 1992
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Galactomannan 1 entry(ies).
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
93
88
Luong et. et., 2011
Galactomannan (ELISA) 61 entry(ies).
Specimen Sensitivity Specificity Link
Serum
100
100
Verweij et al, 1998
Serum
100
93
Kawazu et al, 2004
Serum
100
93
Poirot et al, 1996
Serum
100
89
Tabone et al, 1997
Serum
100
89
Caillot et al, 1997
Serum
100
55
Kawazu et al, 2004
Serum
100
NR
Siemann, 1998
Serum
97.4
90.5
Maertens et al, 2007b
Serum
96.5
98.6
Maertens et al, 2004
Serum
96.5
93.2
Maertens et al, 2004
Serum
95
100
Sabetta et al, 1985
Serum
94.4
98.8
Maertens et al, 2002
Serum
94.4
85.4
Maertens et al, 2002
Serum
93.1
100
Maertens et al, 2004
Serum
92.6
95.4
Maertens et al, 1999
Serum
92.6
95.4
Maertens et al, 1999
Serum
92.6
94.5
Verweij et al, 1995c
Serum
89.7
98.1
Maertens et al, 2001
Bronchoalveolar lavage
88
87
Meersseman et al. (2008)
Serum
87.5
NR
Marr et al, 2004
Bronchoalveolar lavage
85
100
Becker et al, 2003c
Serum
81
89
Wheat, 2003
Serum
80.7
89.2
Platelia Aspergillus
Serum
80.7
89.2
Platelia Aspergillus
Cerebrospinal fluid
80
100
Klont, 2004 (Review article)
Serum
60
82
Maertens et al, 1999
Blood
58
97
Kami et al, 2001
Serum
55.6
93.9
Fortun et al, 2001e
Serum
47
93
Becker et al, 2003c
Urine
44
NR
Klont, 2004 (Review article)
Serum
40
94
Maertens et al, 1999
Serum
30
95
Husain et al, 2004f
Serum
30
93
Husain et al, 2004f
Serum
20
NR
Husain et al, 2004f
Solid tissue
NR
NR
Klont, 2004 (Review article)
Cyst fluid
NR
NR
Klont, 2004 (Review article)
Subphrenic abscess aspirate
NR
NR
Klont, 2004 (Review article)
Pus
NR
NR
Klont, 2004 (Review article)
Serum
NR
NR
Mennink-Kersten, 2003
Solid tissue
NR
NR
Laboratoire de Parasitologie-Mycologie, 2004
Serum
NR
NR
Verweij, 2004 - Review article
Foods
NR
NR
V. Letscher-Bru et al, 1998
Cardboard contamination
NR
NR
Girmenia et al, 2007
Serum
NR
NR
Mennink-Kersten, 2004
Crossreactivity with lipoteichoic acid
NR
NR
Mennink-Kersten, 2004
Crossreactivity with lipoteichoic acid
NR
NR
Mennink-Kersten, 2005
Serum
NR
NR
Sulahian et al, 2003
Serum
NR
NR
Adam et al, 2004
Serum
NR
NR
Maertens et al, 2004
Serum
NR
NR
Kwak et al, 2003
Serum
NR
NR
Maertens et al, 2005
Serum
0
0
Dalle et al., 2005
Serum
0
0
Verweij et al., 2006
Serum
0
0
Wu, 2004
Serum
0
0
Mattei et al., 2004
Serum
0
0
Aubry et al., 2006
Serum
0
0
Sulahian et al., 2003
Urine
0
0
Hashiguchi et al., 1994
Serum
0
0
De Jesus et al., 2007
Serum
0
0
Kappe et al., 1993
Serum
0
0
Giacchino et al., 2006
Specimen Sensitivity Specificity Link
Serum
NR
NR
Weisser et al, 2005
Specimen Sensitivity Specificity Link
Serum
90
84
Verweij et al, 1995
Serum
70
86
Verweij et al, 1995
Serum
67
98.2
Machetti et al, 1998
Serum
50
94
Verweij et al, 1995
Specimen Sensitivity Specificity Link
Plasma
97.6
96.2
Van Cutsem et al, 1990
Serum
90
84
Verweij et al, 1995b
Serum
87
NR
Dupont et al, 1990
Serum
82.5
81
Ansorg et al, 1994
Serum
70
86
Verweij et al, 1995b
Serum
43
53
Vogeser et al, 1997
Serum
38.8
95
Hopwood et al, 1995
Serum
27.5
100
Ansorg et al, 1994
Serum
NR
98.7
Ansorg et al, 1994
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Serum
0
0
PastorexÖ Aspergillus*
Specimen Sensitivity Specificity Link
Serum
95
90
Manso et al, 1994
Galactomannan + PCR 1 entry(ies).
Specimen Sensitivity Specificity Link
Serum
79
61
El-Mahallawy et al, 2006
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
76
100
Musher et al, 2004g
Bronchoalveolar lavage
61
67
Musher et al, 2004g
Specimen Sensitivity Specificity Link
Serum
87.5
100
Pazos et al, 2005.
Serum
87.5
89.6
Pazos et al, 2005
Specimen Sensitivity Specificity Link
Serum
53
NR
Grundy et al, 1995
Halo Sign on CT Scan 1 entry(ies).
Specimen Sensitivity Specificity Link
Halo Sign on CT Scan
33.3
93.3
Maertens et al, 2002
Specimen Sensitivity Specificity Link
Halo Sign on CT Scan
NR
NR
Weisser et al, 2005
Histology 1 entry(ies).
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
53
95
Kahn et al, 1986
Histology+Culture 1 entry(ies).
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
58
92
Kahn et al, 1986
Specimen Sensitivity Specificity Link
0
0
ID-FUNGAL ANTIBODY SYSTEM
Specimen Sensitivity Specificity Link
Paranasal sinus
NR
NR
Fenelon et al, 1999
Brain
NR
NR
Fenelon et al, 1999
Liver
NR
NR
Fenelon et al, 1999
Lung
NR
NR
Fenelon et al, 1999
Specimen Sensitivity Specificity Link
Phagocytes
NR
NR
PiÚrard et al, 1991
Lung
NR
NR
Verweij et al, 2007
Necrotic bone
NR
NR
Verweij et al, 2007
Specimen Sensitivity Specificity Link
Lung
NR
NR
Choi et al, 2004
Nasopharynx
NR
NR
Choi et al, 2004
Spleen
NR
NR
Choi et al, 2004
Brain
NR
NR
Choi et al, 2004
Heart
NR
NR
Choi et al, 2004
Ear
NR
NR
Choi et al, 2004
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
Specimen Sensitivity Specificity Link
Cerebrospinal fluid
NR
NR
Kappe et al, 1996
Serum
NR
NR
Kappe et al, 1996
PCR 12 entry(ies).
Specimen Sensitivity Specificity Link
Blood
100
84
Herbart et al, 2000b
Blood
100
73
Hebart et al, 2000
Bronchoalveolar lavage
93.9
94.4
Buchheidt et al, 2002
Serum
92.3
94.6
White et al, 2006
Bronchoalveolar lavage
90
100
Sangunetti et al (2003)
Bronchoalveolar lavage
80
93
Raad et al, 2002
Serum
75
92
El-Mahallawy et al, 2006
Serum & Plasma
0.75
0.87
Mengoli et al, 2009
Blood
NR
100
Loeffler, 2002
Bronchoalveolar lavage
NR
100
Loeffler, 2002
Blood
NR
NR
Loeffler, 2001
Bronchoalveolar lavage
NR
NR
Spreadbury et.al. (1993)
PCR-ELISA 3 entry(ies).
Specimen Sensitivity Specificity Link
Lung
78
NR
Loeffler et al, 2002b
Serum
63.6
89.7
Florent et al, 2006
Blood
25
NR
Loeffler et al, 2002b
Specimen Sensitivity Specificity Link
Positron Emission Tomography
NA
NA
Aspergillus Website, 2008
qPCR 2 entry(ies).
Specimen Sensitivity Specificity Link
Blood
100
100
Loeffler et al, 2000
Bronchoalveolar lavage
67
100
Musher et al, 2004g
qPCR+Galactomannan 1 entry(ies).
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
82
96
Musher et al, 2004g
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
85
96
Luong et. et., 2011
Specimen Sensitivity Specificity Link
Bronchoalveolar lavage
100
88
Luong et. et., 2011
Specimen Sensitivity Specificity Link
Lung
NR
NR
Kaufman et al, 1997
Brain
NR
NR
Kaufman et al, 1997
Kidney
NR
NR
Kaufman et al, 1997
Maxillary sinus
NR
NR
Kaufman et al, 1997
Skin
NR
NR
Kaufman et al, 1997
Radioimmune assay 1 entry(ies).
Specimen Sensitivity Specificity Link
Serum + Urine + BAL
75
90
Maertens et al, 2007
Specimen Sensitivity Specificity Link
Serum
NR
NR
Dupont et al, 1987
Urine
NR
NR
Dupont et al, 1987
rtPCR 8 entry(ies).
Specimen Sensitivity Specificity Link
Blood
79
92
Kami et al, 2001
Blood
67
84
Kami et al, 2001
Serum
45
98
Kawazu et al, 2004
Serum
36
100
Kawazu et al, 2004
Blood
0
0
LightCycler«SeptiFast Test
Whole blood, serum and plasma
0
0
affigene« Aspergillus tracer
blood, blood cells, culture cells, plasma, serum, sputum, stool, plant and animal tissue and food
0
0
Automated nucleic acid purification
Respiratory specimen and serum
0
0
MycAssay Aspergillus
Specimen Sensitivity Specificity Link
Culture Media
NR
NR
Etest Amphotericin B
Culture Media
NA
NA
Etest Anidulafungin
Culture Media
0
0
Etest Caspofungin
Culture Media
0
0
Etest Fluconazole
Culture Media
0
0
Etest Flucytosine
Culture Media
0
0
Etest Itraconazole
Culture Media
0
0
Etest Ketoconazole
Culture Media
0
0
Etest Posaconazole
Culture Media
0
0
Etest Voriconazole
Culture Media
0
0
Etest Fluconazole
Culture Media
0
0
Etest Flucytosine
Culture Media
0
0
Etest Itraconazole
Culture Media
0
0
Etest Posaconazole
Culture Media
0
0
Etest Anidulafungin
Culture Media
0
0
Etest Caspofungin
Culture Media
0
0
Etest
▀-Glucan 25 entry(ies).
Specimen Sensitivity Specificity Link
Serum
100
90
Odabasi et al, 2004j
Serum
100
90
Odabasi et al, 2004
Serum
100
NR
Maertens et al, 2007
Serum
100
0
Fungitell assay
Serum
100
0
Fungitell assay
Serum + Plasma
93.3
77.2
Pickering et al, 2005
Serum
92.9
100
Pickering et al, 2005
Serum
90
100
Maertens et al, 2007
Plasma
90
100
Obayashi et al, 1995
Serum
87.5
89.6
Pazos et al, 2005
Serum
87.5
89.6
Pazos et al, 2005.
Serum
69.9
87.1
Ostrosky-Zeichner et al, 2005j
Serum
69.9
87.1
Ostrosky-Zeichner et al, 2005
Serum
65
96
Odabasi et al, 2004j
Serum
65
96
Odabasi et al, 2004
Serum
64.4
92.4
Ostrosky-Zeichner et al, 2005j
Serum
64.4
92.4
Ostrosky-Zeichner et al, 2005
Serum
60
99
Odabasi et al, 2004j
Serum
60
99
Odabasi et al, 2004
Serum
55
98
Kawazu et al, 2004
Serum
45
99
Kawazu et al, 2004
Serum
0
0
FungiTec G
Serum
0
0
Gluspecy
Serum
0
0
Beta-D-glucan Test Wako
Serum
0
0
B-G Star
Specimen Sensitivity Specificity Link
Serum
87.5
100
Pazos et al, 2005
Testing kits
 
Approved for EU (8 categories).
Specimen Test Description
Approved for use in:
Manufacturer Reference
A. fumigatus Complement A. fumigatus Complement Fixation Reagents
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Specimen Test Description
Approved for use in:
Manufacturer Reference
Aspergillus Immunodiffusion Aspergillus Immunodiffusion Group Species
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Aspergillus Immunodiffusion Aspergillus Immunodiffusion Individual Species
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Galactomannan (ELISA) 1 entry(ies).
Specimen Test Description
Approved for use in:
Manufacturer Reference
Serum Galactomannan (ELISA) Platelia Aspergillus
EU
Biorad
Specimen Test Description
Approved for use in:
Manufacturer Reference
Serum Galactomannan (Latex agglutination) PastorexÖ Aspergillus*
EU
Biorad
Specimen Test Description
Approved for use in:
Manufacturer Reference
ID-FUNGAL ANTIBODY SYSTEM ID-FUNGAL ANTIBODY SYSTEM
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
rtPCR 4 entry(ies).
Specimen Test Description
Approved for use in:
Manufacturer Reference
Blood rtPCR LightCycler«SeptiFast Test
EU
Roche
Whole blood, serum and plasma rtPCR affigene« Aspergillus tracer
EU
Affigene
blood, blood cells, culture cells, plasma, serum, sputum, stool, plant and animal tissue and food rtPCR Automated nucleic acid purification
EU (Investigational use only)
Roche Applied Science
Respiratory specimen and serum rtPCR MycAssay Aspergillus
EU
Myconostica
Specimen Test Description
Approved for use in:
Manufacturer Reference
Culture Media Susceptibility testing (MIC) Etest Amphotericin B
EU
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Anidulafungin
EU (Investigational use only)
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Caspofungin
EU (In Vitro Diagnostic)
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Fluconazole
EU
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Flucytosine
EU
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Itraconazole
EU
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Ketoconazole
EU
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Posaconazole
EU (In Vitro Diagnostic)
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Voriconazole
EU
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest
EU & USA
AB Biodisk
▀-Glucan 1 entry(ies).
Specimen Test Description
Approved for use in:
Manufacturer Reference
Serum ▀-Glucan Fungitell assay
EU
Associates of Cape Cod Inc.
 
Approved for USA (6 categories).
Specimen Test Description
Approved for use in:
Manufacturer Reference
A. fumigatus Complement A. fumigatus Complement Fixation Reagents
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Specimen Test Description
Approved for use in:
Manufacturer Reference
Aspergillus Immunodiffusion Aspergillus Immunodiffusion Group Species
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Aspergillus Immunodiffusion Aspergillus Immunodiffusion Individual Species
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Galactomannan (ELISA) 1 entry(ies).
Specimen Test Description
Approved for use in:
Manufacturer Reference
Serum Galactomannan (ELISA) Platelia Aspergillus
USA
Bio-Rad Laboratories
Specimen Test Description
Approved for use in:
Manufacturer Reference
ID-FUNGAL ANTIBODY SYSTEM ID-FUNGAL ANTIBODY SYSTEM
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Specimen Test Description
Approved for use in:
Manufacturer Reference
Culture Media Susceptibility testing (MIC) Etest Fluconazole
USA
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Flucytosine
USA
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Itraconazole
USA
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Posaconazole
USA (Investigational Use Only)
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Anidulafungin
USA (Investigational Use Only)
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest Caspofungin
USA (Investigational Use Only)
AB Biodisk
Culture Media Susceptibility testing (MIC) Etest
EU & USA
AB Biodisk
▀-Glucan 1 entry(ies).
Specimen Test Description
Approved for use in:
Manufacturer Reference
Serum ▀-Glucan Fungitell assay
USA
Associates of Cape Cod Inc.
 
Approved for Japan (1 categories).
▀-Glucan 4 entry(ies).
Specimen Test Description
Approved for use in:
Manufacturer Reference
Serum ▀-Glucan FungiTec G
Japan
Seikagaku Corporation
Serum ▀-Glucan Gluspecy
Japan
Seikagaku Corporation
Serum ▀-Glucan Beta-D-glucan Test Wako
Japan
Wako Pure Chemical Industries (Tokyo, Japan)
Serum ▀-Glucan B-G Star
Japan
Maruha Corporation (Tokyo, Japan)
Other
 
Reagents and Equipment (11 categories).
Test Description
Approved for use in:
Manufacturer Reference
A. fumigatus Complement A. fumigatus Complement Fixation Reagents
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Test Description
Approved for use in:
Manufacturer Reference
Antibody IgA, IgE, IgG Galactomannan Labor Diagnostics
Labor Diagnostics
Test Description
Approved for use in:
Manufacturer Reference
Antibody IgG, IgM Galactomannan Roche Serologie GmbH, Munich
Roche-Serologie
Test Description
Approved for use in:
Manufacturer Reference
Antibody IgM(IgG) Galactomannan Kappe, 1996
Fumouze Diagnostics, France
Test Description
Approved for use in:
Manufacturer Reference
Antibody Mab 164G & 611F Asp. species specific Fenelon et al., 1999
Fenelon et al
Test Description
Approved for use in:
Manufacturer Reference
Antibody Mab IgM Aspergillus Aspergillus antibodies (x5)
Santa Cruz biotechnology, Inc
Test Description
Approved for use in:
Manufacturer Reference
Antibody Mab-WF-AF-1 Galactomannan Choi et al., 2004
Dako
Test Description
Approved for use in:
Manufacturer Reference
Aspergillus antibodies Aspergillus antibodies (x32)
Antibodies-online.com
Aspergillus antibodies Aspergillus antibodies (x9)
Acris Antibodies
Test Description
Approved for use in:
Manufacturer Reference
Aspergillus Immunodiffusion Aspergillus Immunodiffusion Group Species
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Aspergillus Immunodiffusion Aspergillus Immunodiffusion Individual Species
EU & USA(in vitro diagnostic use only)
Immuno Mycologics, Inc
Test Description
Approved for use in:
Manufacturer Reference
Positron Emission Tomography Aspergillus Website, 2008
rtPCR 3 entry(ies).
Test Description
Approved for use in:
Manufacturer Reference
rtPCR LightCycler«SeptiFast Test
EU
Roche
rtPCR affigene« Aspergillus tracer
EU
Affigene
rtPCR Automated nucleic acid purification
EU (Investigational use only)
Roche Applied Science
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